Mechanism of Action Miconazole inhibits 14-a-demethylase, a microsomal cytochrome P-450-dependent enzyme system critical to sterol synthesis. Accumulation of sterols with a 14-a-methyl
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چکیده
Miconazole is widely used in prescription and non-prescription products, including extensive use for vaginal yeast infections; in addition to medical use, azole molecules are widely used in agriculture. Despite this widespread use, the only known circumstances in which resistance to azole antifungal agents has developed conspicuously, unequivocally and measurably is among HIV-infected individuals treated with fluconazole for oral Candida infections.
منابع مشابه
Cytochrome P-450-dependent oxidation of lanosterol in cholesterol biosynthesis. Microsomal electron transport and C-32 demethylation.
Electron transfer to rat liver microsomal cytochrome P-450 of 14 alpha-methyl group demethylation of 24,25-dihydrolanosterol (C30-sterol) has been studied with a new radio-high-performance liquid chromatography assay. The monooxygenase is dependent upon NADPH plus oxygen, insensitive to CN-, and sensitive to CO. Microsomal oxidation is also sensitive to trypsin digestion, and reactivation is de...
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